15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms
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چکیده
منابع مشابه
Association of apolipoprotein E4 and haplotypes of the apolipoprotein E gene with lobar intracerebral hemorrhage.
BACKGROUND AND PURPOSE Conflicting reports in the literature exist with regard to the association of apolipoprotein E (apo E) alleles and lobar intracerebral hemorrhage (ICH). We genotyped 12 single-nucleotide polymorphisms in the 5' upstream regulatory, exonic, and intronic regions of the apo E gene and performed genotype and haplotype association analyses. METHODS We prospectively enrolled ...
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Tadafumi Hashimoto,1 Alberto Serrano-Pozo,1 Yukiko Hori,1 Kenneth W. Adams,1 Shuko Takeda,1 Adrian Olaf Banerji,1 Akinori Mitani,1 Daniel Joyner,1 Diana H. Thyssen,1 Brian J. Bacskai,1 Matthew P. Frosch,1 Tara L. Spires-Jones,1 Mary Beth Finn,2 David M. Holtzman,2 and Bradley T. Hyman1 1Department of Neurology, Alzheimer’s Disease Research Unit, Massachusetts General Hospital, Charlestown, Mass...
متن کاملApolipoprotein E, especially apolipoprotein E4, increases the oligomerization of amyloid β peptide.
Alzheimer's disease (AD) is the most common progressive neurodegenerative disorder causing dementia. Massive deposition of amyloid β peptide (Aβ) as senile plaques in the brain is the pathological hallmark of AD, but oligomeric, soluble forms of Aβ have been implicated as the synaptotoxic component. The apolipoprotein E ε 4 (apoE ε4) allele is known to be a genetic risk factor for developing AD...
متن کاملIntroduction of human apolipoprotein E4 "domain interaction" into mouse apolipoprotein E.
Human apolipoprotein E4 (apoE4) binds preferentially to lower density lipoproteins, including very low density lipoproteins, and is associated with increased risk of atherosclerosis and neurodegenerative disorders, including Alzheimer's disease. This binding preference is the result of the presence of Arg-112, which causes Arg-61 in the amino-terminal domain to interact with Glu-255 in the carb...
متن کاملA liquid chromatography-mass spectrometry method for the quantification of urinary albumin using a novel 15N-isotopically labeled albumin internal standard.
and leukocyte count and the CYP2J2 genotype. The CAD status was included as main effect, and statistical adjustments were made for age, sex, and cardiovascular risk factors (body mass index, diabetes mellitus, hypertension, and smoking). None of these models revealed any statistically significant relationships between the CYP2J2 genotype and inflammatory markers (data not shown). In contrast to...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2012
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac3018873